ABSTRACT
Objective To analyze the correlation between three evaluation methods of middle cerebral artery occlu -sion and focal cerebral ischemia , and to explore a new method and standard for the evaluation of the model .Method Thirty healthy adult male SD rats were randomly divided into a sham-operated group ( n=20 ) and model group .According to Zea-Longa procedure ,we established the disease model to detect the changes in cerebral blood flow before and after sur -gery.The resulted cerebral infarction area was taken as gold standards .Then we analyzed the correlation between the standards and the changes in cerebral blood flow .Results The changes of infarction area ratio were positively correlated with the changes of cerebral blood flow in the model rats .Conclusions The changes of rat cerebral blood flow can be used to evaluate whether the cerebral ischemia model is successful or not .
ABSTRACT
This study is to investigate the treatment of Jin Chai antiviral capsule for influenza virus FM1/47 (H1N1) infection. The model of pneumonia was established by dropping influenza virus into the nose of normal mice, real-time PCR and Western blot technique were used to detect the virus load and the interferoninducible transmembrane protein3 (IFITM3) in lung of mice at the 1st day, 3rd day, 5th day and 7th day after affected. The results showed that Jin Chai antiviral capsule in large, middle, small dose groups can decrease virus load significantly at each time point, after being affected (P<0.05, P<0.01), Jin Chai antiviral capsule can increase the interferoninducible transmembrane protein3 in lung of mice, large dose groups are significantly higher in expression of IFITM3 compared with model group at each time point (P<0.05, P<0.01). Middle dose groups are significantly higher in expression of IFITM3 compared with model group at the 3th day and the 5th day (P<0.05), small dose groups are significantly higher in expression of IFITM3 compared with model group at the 3th day (P<0.05). It can be concluded that Jin Chai antiviral capsule exerts antiviral effects against influenzavirus by raised expression of IFITM3.